农业微生物学

　《微生物学》试题 Ⅰ

　本试题一共 2 道大题，共 2 页，满分 100 分。考试时间 120 分钟。

　总 分

　 题号 一 二 三 四 五 六 七 八 九 十 阅卷人

　 题分

　 核分人

　 得分

　 注：

　1. 答题前，请准确、清楚地填各项，涂改及模糊不清者、试卷作废。

　2. 试卷若有雷同以零分计。

　一、名词解释(6×5)：

　1. Colony 2. Virus 3. Biogeochemical cycle 4. Medium 5. Tansduction 6. Mycorhiza 二、问答题(70 分) 1.What are the major differences between gram-positive and Gram-negative bacteria？(5 分) 2.Distinguish between (a) phototrophs and chemotrophs, (b) Lithotrophs and organotrophs. (4 分)

　3.Yeast can grow both under aerobic and anaerobic conditions. What is the difference in energy production? (8 分) 4.Draw a typical bacteria growth curve. Explain each phase. (8 分)In what circumstance, filtration and pasteurization methods are useful? (6 分)

　5.To determine the viable counts of a soil sample, which method is the best? List the procedures.(10 分)

　6.The donor bacterial cell in a transformation is probably dead. Explain why. (2 分)

　7.What are the roles of a cloning vector, restriction enzymes, and DNA ligase in molecular cloning? (6 分)

　8.What does the term “antagonism” means? Explain the usefulness in biological control. (4 分)

　9.Explain the following terms in Rhizobium-legume symbiosis: infection, host specificity, effectiveness, competitiveness. How to evaluate the symbiotic effectiveness in green-house? (10 分) 10.List the taxonomic hierarchy of classification. (7 分)

　《微生物学》试题 Ⅱ

　本试题一共 2 道大题，共 2 页，满分 100 分。考试时间 120 分钟。

　总 分

　 题号 一 二 三 四 五 六 七 八 九 十 阅卷人

　 题分

　 核分人

　 得分

　 注：

　1. 答题前，请准确、清楚地填各项，涂改及模糊不清者、试卷作废。

　2. 试卷若有雷同以零分计。

　一、名词解释(6×5)：

　1. Bacterial chemotaxis 2. Lichen 3. Chemoorganotroph

　4. Colony 5. Biogeochemical cycle

　6. Conjugation 二、问答题(70 分) 1.What are the unique features of microorganisms gram-positive？ (10 分) 2.Why does Gram-staining procedure stain some bacteria purple-violet and others red?(10 分) 3.Explain the term “medium”,what is the purpose to make a selective medium? (10 分)

　4.To determine the viable counts of a soil sample, which method is the best? List the procedures. (8 分) 5.What are the roles of a cloning vector,restriction enzyme and DNA Ligase in molecular cloning? (12 分)

　6.Explain the following terms in Rhizobium-legume symbiosis: infection, host specificity, effectiveness, competitiveness. How to evaluate the symbiotic effectiveness in green-house? (20 分)

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